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Product Info Summary
| SKU: | LS005049 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa) (PAFAH1B2) |
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Product info
Product Name
PAFAH1B2 (NM_002572) Human Over-expression Lysate
SKU/Catalog Number
LS005049
Size
100 µg
Description
Transient overexpression lysate of platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa) (PAFAH1B2)
Storage & Handling
Cite This Product
PAFAH1B2 (NM_002572) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005049)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PAFAH1B2 Human Over-expression Lysates NM_002572.
Western blot validation of overexpression lysate (LS005049) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PAFAH1B2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PAFAH1B2 (Source: Uniprot.org, NCBI)
Gene Name
PAFAH1B2
Full Name
Platelet-activating factor acetylhydrolase IB subunit beta
Weight
25.569kDa
Superfamily
GDSL' lipolytic enzyme family
Alternative Names
EC 3.1.1.47; intracellular platelet-activating factor acetylhydrolase alpha 2 subunit; PAF acetylhydrolase 30 kDa subunit; PAF-AH 30 kDa subunit; PAFAH subunit beta; PAF-AH subunit beta; PAF-AH1b alpha 2 subunit; PAFAHB; platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa); platelet-activating factor acetylhydrolase IB subunit beta; platelet-activating factor acetylhydrolase, isoform Ib, beta subunit 30kDa; platelet-activating factor acetylhydrolase, isoform Ib, subunit 2 (30kDa) PAFAH1B2 HEL-S-303 platelet activating factor acetylhydrolase 1b catalytic subunit 2 platelet-activating factor acetylhydrolase IB subunit alpha2|PAF acetylhydrolase 30 kDa subunit|PAF-AH 30 kDa subunit|PAF-AH subunit beta|PAF-AH1b alpha 2 subunit|PAFAH subunit beta|epididymis secretory protein Li 303|epididymis secretory sperm binding protein|intracellular platelet-activating factor acetylhydrolase alpha 2 subunit|platelet-activating factor acetylhydrolase 1b, catalytic subunit 2 (30kDa)|platelet-activating factor acetylhydrolase IB subunit beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PAFAH1B2, check out the PAFAH1B2 Infographic
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In this infographic, you will see the following information for PAFAH1B2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PAFAH1B2 (NM_002572) Human Over-expression Lysate (LS005049)
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